Mouse Kidney Cytidine Deaminase
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چکیده
A nucleoside aminohydrolase was prepared in 60-fold relative purity from the nonparticulate fraction of mouse kidney by denaturation at 60”, fractionation with (NH&S04, treatment with alumina gel Cr (pH 7.5), and dialysis in 0.02 M acetate buffer (pH 4.5). The’ optimal pH was 6.5. Cytidine, deoxycytidine, and I-/3-D-arabinofuranosylcytosine were deaminated, and the respective K, values were 7.53 x 10-5, 7.25 x 10d5, and 1.01 x 10W3. Divalent metals were not required for activity. Inhibition by p-chloromercuribenzoate indicated participation of sulfhydryl groups.
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تاریخ انتشار 2003